1 A PRIMER ON TRANSCRIPTIONAL REGULATION IN MAMMALIAN CELLS 2 INITIAL STRATEGIC ISSUES 3 MODES OF REGULATING mRNA ABUNDANCE 4 TRANSCRIPTION INTIATION SITE MAPPING 5 FUNCTIONAL ASSAYS FOR PROMOTER ANALYSIS 6 IDENTIFICATION AND ANALYSIS OF DISTANT CONTROL REGIONS 7 IDENTIFYING cis-ACTING DNA ELEMENTS WITHIN A CONTROL REGI N_8 IDENTIFICATION OF DNA-BINDING PROTEINS AND ISOLATION OF THEIR GENES 9 CONFIRMING THE FUNCTIONAL IMPORTANCE OF A PROTEIN-DNA INTERACTI N_10 IN VIVO ANALYSIS OF AN ENDOGENOUS CONTROL REGI N_11 APPROACHES FOR THE SYNTHESIS OF RECOMBINANT TRANSCRIPTION FACTORS 12 IDENTIFYING AND CHARACTERIZING TRANSCRIPTION FACTOR DOMAINS 13 THEORY,CHARACTERIZATION,AND MODELING OF DNA BINDING BY REGULATORY TRANSCRIPTION FACTORS 14 CRUDE AND FRACTIONATED SYSTEMS FOR IN VITRO TRANSCRIPTI N_15 APPROACHES FOR STUDYING TRANSCRIPTION COMPLEX ASSEMBLY APPENDICES INDEX