Preface Abbreviations CHROMOSOMES 1 Preparation and Analysis of Drosophila Mitotic Chromosomes 2 In Situ Hybridization to Somatic Chromosomes 3 BrdU Labeling of Chromosomes 4 Analysis of Meiosis of Fixed and Live Oocytes by Light Microscopy 5 Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster 6 Preparation and Analysis of Polytene Chromosomes 7 In Situ Hybridizaiton to Polytene Chromosomes 8 Mapping Protein Distributions on Polytene Chromosomes by Immunostaining CELL BIOLOGY 9 Fluorescent Analysis of Drosophila Embryos 1 Imaginal Discs 11 The Neuromuscular Junction 12 Histological Techniques for the Drosophila Eye.Part Ⅰ:Larva and Pupa 13 Histological Techniques for the Drosophila Eye.Part Ⅱ:Adult 14 Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy 15 Electrophysiological Approaches to the Neuromusculature 16 Functional Assays of the Peripheral and Central Nervous Systems 17 GFP and Other Reporters 18 Quantitative Microinjection of Drosophila Embryos 19 Targeted Disruption of Gene Function in Drosophila by RNA Interference 2 Drosophila Cell Culture and TRransformation 21 Generating Antibodies against Drosophila Proteins 22 Photoactivated Gene Expression for Cell-fate Mapping and Cell Manipulation MOLECULAR BIOLOGY 23 Recovery of DNA Sequences Flanking P-element Insertions:Inverse PCR and Plasmid Rescue 24 GAL4-mediated Ectopic Gene Expression in Drosophila 25 Functional Cell Ablation GENOMICS 26 Preparation of DNA from E.coli Cells Containing Bacteriophage P1 Clones 27 Drosophila Microarrays:From Arrayer Construction to Hybridization 28 Using Drosophila Genome Databases 29 Preparation and Analysis of High-molecular-weight DNA in Drosophila BIOCHEMISTRY 3 Culturing Large Populations of Drosophila for Protein Biochemistry 31 Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis 32 Preparation of Membrane Proteins from Drosophila Embryos 33 Preparing Cytoplasmic Extracts from Drosophila Embryos 34 Immunoblotting of Proteins from Single Drosophila Embryos THE ORGANISM 35 Laboratory Culture of Drosophila 36 Preparation of Larval and Adult Cuticles for Light Microscopy 37 Exposing Drosophila to Neuroactive Drugs APPENDICES 1 1989 Table of Contents from Drosophila:A Laboratory Manual 2 Anatomical Drawings of Drosophila 3 Solutions and Reagents 4 Cautions 5 Suppliers 6 Trademarks Index
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