Experiment 1 Preparation of Plasmid DNA Experiment 2 Restriction Enzyme Digestion and Agarose Gel Electrophoresis Experiment 3 Polymerase Chain Reaction（PCR） Experiment 4 DNA Purification and Construction of Recombinant DNA Experiment 5 Preparation of Competent E.coli Cells Experiment 6 Transformation of Recombinant DNA Experiment 7 Identification and Characterization of Positive Clones Experiment 8 Isolation ofTotal RNA from Animal Tissues Experiment 9 Molecular Hybridization Experiment 10 Reverse Transcription—Polymerase Chain Reaction（RT-PCR） and TA-cloning Experiment 11 Expression and Purification of a Fusion Protein from E.coli Experiment 12 Electrophoretic Mobility Gel Shift Assay（EMSA）to Detect DNA—Protein Interactions Appendix I Brief Introduction to the NCBI Database Appendix II Vector Information Appendix III Instructions on How to Use the Equipment References
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